Objectives and Content

The course is experiment-oriented and includes selected basic methods in physical biochemistry, biochemical analysis and separation methods, gene technology, immunology and microscopy. The course includes exercises in spectrophotometry, chromatography, enzymology, electrophoresis, isolation of biological macromolecules, in situ hybridisation, immunological detection methods and other important methods in gene technology. Work with different biological systems will be emphasised. Organisation of the laboratory work and combining use of different methods for analysis of specific issues will be important. Safety in the laboratory environment and journal writing will also be covered. http://www.uib.no/en/course/MOL300MOL300 is compulsory for the Master's Programme in Molecular Biology.

Knowledge:

The student

• can explain the theories underlying both basic and some advanced methods in biochemistry and molecular biology
• can explain advantages and disadvantages of the most important methods in biochemistry and molecular biology
• can work with DNA, RNA and proteins
• is familiar with the appropriate laboratory safety regulations both with regard to personal and environmental aspects.

Skills:

The student

• can use a broad range of both basic and some advanced methods in biochemistry and molecular biology (including chromatography, electrophoresis, spectroscopy, enzyme kinetics, molecular cloning, cell culture work and microscopy)
• can examine, assess, interpret and communicate data acquired from laboratory experiments related to biochemistry and molecular biology
• can keep a detailed and accurate laboratory journal

General competence:

The student

• can do experimental work related to biochemistry and molecular biology without detailed instruction and supervision.

Lectures, laboratory work, journal and report. The attendance and the participation in all activities including the

first day meeting are compulsory.

Approved lab-journal and written report (30 %). Written exam 5 hours (70 %).

Examination support materials: Non- programmable calculator, according to model listed in faculty regulations

MOL302 15sp