Spring

The course will only be given if at least 4 students register for the  course and is limited to 10 students.

Department of Clinical Science,

Lab.building/ BB-building & Haukeland University Hospital.

Aim:

Basic introduction to flow cytometry. The practical part of the course should enable the students to understand applications for flow cytometry. In addition, the applications used during the practical part of the course will be introduced in seminars by the course leaders.

Content:

Theoretical part:

- The course leaders will give a theoretical introduction to flow cytometry and some of the most common applications.

Practical part: Hands-on training at one of the local flow cytometers (BD accuri)

- Quantification of apoptotic cells

- Cell cyclus analyses

- Simple staining of cells with surface markers

- Analysis of transfected cells

Knowledge

Upon completing the course, the participants should

- be familiar with basic applications for flow cytometry

- know which and how many different fluorochromes one can use on a given flow cytometer

- be able to evaluate the quality of flow cytometry data

Skills

Upon completing the course, the participants should be able to

- plan and perform basic experiments using flow cytometry

- quality control generated flow cytometry data

- present flow cytometry data in a scientific way

Competence

Upon completing the course, the participants should

- have basic understanding of flow cytometry

- understand why to use flow cytometry in a certain research question

- be able to interpret basic flow cytometry data

Written lab report (composed of Introduction, Materials and Methods, Results, Discussion).

Has to be approved by the course leader.

Flow cytometry - A Basic Introduction, Wiki version;

Michael Ormerod.

Course coordinator: Silke Appel Phone: 559 74633, E-mail: Silke.Appel@uib.no

Adm. E-mail: studie@kliniskmedisin.uib.no or Elin.Heggernes@uib.no

Department of Clinical Science