The course consists of two parts:

Spectroscopy, 2 sp

Biochemical methodology, 1 sp

Spectroscopy:

This part entails spectroscopic techniques used in the analysis of organic compounds. Infrared (IR) and ultraviolet (UV) spectroscopy will be discussed, while the main focus will be on nuclear magnetic resonance (NMR) and mass spectrometry (MS). Students will learn how to identify unknown compounds by using information from IR, UV, MS and NMR spectra.

Relevant pharmaceutical examples will be discussed.

Biochemical methodology:

Biochemical Methodology covers the theory behind and practical implementation of some basic techniques in molecular biology and biochemistry. The main focus is on purification of plasmids, transformation of these into competent bacteria, PCR, use of restriction enzymes, SDS-PAGE and western/spot blotting (recognition of native protein with antibody). The course also covers selected topics of applied bioinformatics (important databases for DNA and protein sequences, sequence alignments, sequence similarity searches, and homology modelling, including AlphaFold).

Spectroscopy

Explain/discuss the different spectroscopic techniques (IR, UV/vis, NMR and MS) and interpret spectroscopic data from IR, UV/vis, NMR and MS either by themselves of combinations of these in order to determine the structures of organic compounds.

Biochemical methodology

• Master the basic knowledge behind the practical methods that are used in this course, be able to perform basic cloning techniques, PCR and to express recombinant protein in bacteria and to perform the SDS gel electrophoresis and Western / spot blot techniques. The student should be able to master basic molecular biological techniques in general and be able to carry out similar experiments
• Know about important databases to find information about protein and DNA sequences
• Master the basic knowledge behind the computational methods that are used in the course (searches for DNA and protein sequences in databases, sequence alignments, homology modelling)

Spectroscopy: http://www.uib.no/nb/emne/FARM103FARM103, http://www.uib.no/nb/emne/FARM110FARM110, http://www.uib.no/nb/emne/FARM130FARM130, http://www.uib.no/nb/emne/FARM131FARM131, http://www.uib.no/nb/emne/FARM250FARM250, http://www.uib.no/nb/emne/FARM150FARM150, http://www.uib.no/nb/emne/FARM260FARM260, http://www.uib.no/nb/emne/MAT101MAT101

Biochemical methodology: http://www.uib.no/nb/emne/FARM150FARM150

It is mandatory to be present and participate in the biochemical methodology part of the course, in addition to a compulsory submission of an independently carried out bioinformatics exercise.

The biochemical methodology part must be passed in order to sit for the exam in Spectroscopy. Compulsory activity is valid for five semesters.

Spectroscopy: Written exam (2.5 hours). The total time inlcudes uploading of files.

Permitted examination support materials, The textbook (Williams and Fleming: Spectroscopic methods in organic chemistry 6th Edition.). It is not allowed to have notes in the textbook, which can be used in the exam. Underlining of text, figures and tables in the textbook are allowed.